Fig. 6: Single nucleotide incorporation assay with 7-deaza modified dB templates. | Nature Communications

Fig. 6: Single nucleotide incorporation assay with 7-deaza modified dB templates.

From: A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase

Fig. 6: Single nucleotide incorporation assay with 7-deaza modified dB templates.

a Schematic representation of modified B and possible B:U mismatch pairs. B (enol) tautomer:U pair and B:U (enol) tautomer were shown. Note that B:S and B:U (enol) pairs are modeled in our final structures. b Time-course of a single nucleotide incorporation assay: 100 μM of each nucleoside triphosphate was added and results were recorded in 0 s, 15 s, 1 min, 5 min and 30 min, respectively. Each reaction is repeated independently for three times (n = 3). The band of time 0 s (9mer) serves as internal molecular weight marker: p32-labeled 9mer RNA (5’-AUGGAGAGG-3’).

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