Table 1 Kinetic parameters of natural/unnatural base incorporation

From: A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase

 

Kpol (sec-1)

Kd_app (μM)

Kpol / Kd_app (sec-1μM-1)

Relative Efficiency

dT: ATP

9.4 ± 0.3

0.10 ± 0.01

92 ± 11

83

dS: BTP

6.3 ± 0.2

5.7 ± 0.9

1.1 ± 0.2

1

dS: ATP

1.8 ± 0.1

270 ± 40

(6.6 ± 1.0) * 10-3

0.006

dB: STP

5.8 ± 0.3

15 ± 2

0.39 ± 0.07

0.35

dB: UTP

9.1 ± 0.2

12 ± 1

0.79 ± 0.09

0.72

  1. The initial velocities were derived from time-course measurements conducted at various substrate concentrations. The kinetic parameters were obtained by plotting these initial velocities against substrate concentrations and fitting the data to the Michaelis-Menten equation.
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