Fig. 4: DAFS variants retain Vt interactions with F-actin and PIP2.
From: Molecular basis and cellular functions of vinculin-actin directional catch bonding
a High speed F-actin co-sedimentation assays comparing actin binding of WT Vt, DAFS and the actin-deficient I997A Vt variant. Vt I997A is an actin-binding deficient control. b Low-speed F-actin co-sedimentation assays with WT Vt, DAFS and Vt I997A variants comparing the fraction of F-actin present in bundles or in higher-order assemblies. Box-whisker plots with n = 12 independent measurements pooled from N = 3 preparations are presented for F-actin a binding and b bundling co-sedimentation assays, respectively. c Box-whisker plot with n = 7 independent measurements from N = 2 preparations comparing WT Vt and DAFS Vt variant association with PIP2-containing LUVs. Box depicts the median as the center value, the 25th percentile as the lower bound, and the 75th percentile as the upper bound. Whiskers extend 1.5 times the interquartile region (IQR) from the bottom and top of box, or to the minimum and maximum of the data if the data does not extend to the whiskers. Values outside the whiskers are plotted as individual points. One-way analysis of variance (ANOVA) and Tukey’s HSD test were used for statistical analysis. Different letters denote significant differences at p < 0.05. See Supplementary Tables 1–3 for a detailed listing of p-values. Source data are provided as a Source Data file.
