Fig. 5: Vcn DAFS variants do not display altered FA characteristics.
From: Molecular basis and cellular functions of vinculin-actin directional catch bonding
a–d Representative images of Vcn−/− MEFs expressing WT VcnVenus or VcnVenus containing DAFS variants. Scale bar: 10 μm. WT VcnVenus is shown in gray and VcnVenus DAFS variants are shown in shades of blue. Quantification of FA morphometric characteristics are shown including e, FA number, f, FA area, g FA axis ratio, and h, standard deviation of FA orientation. Plots shown for WT VcnVenus and VcnVenus DAFS variants (n = 45, 52, 59, 51 cells, respectively, collected over N = 3 independent experiments). Box depicts the median as the center value, the 25th percentile as the lower bound, and the 75th percentile as the upper bound. Whiskers extend 1.5 times the IQR from the bottom and top of box, or to the minimum and maximum of the data if the data does not extend to the whiskers. Values outside the whiskers are plotted as individual points. One-way ANOVA paired with a Steel-Dwass test (e, f, g) or a Tukey’s HSD test (h) was conducted across all VcnVenus, VcnCS, and VcnTS constructs used for statistical analyses. Differing letters denote significant difference at p < 0.05. For more than two comparisons, see Supplementary Tables 4–7 for a detailed listing of p-values. Source data are provided as a Source Data file.
