Fig. 8: Local FA-SF alignment and directed migration ability differ between WT VcnTS and DAFS double variant VcnTS.
From: Molecular basis and cellular functions of vinculin-actin directional catch bonding
a VcnTS or b, DAFS double variant VcnTS E1015A-E1021A stained for F-actin (purple). Scale bar (and zoom-in height and width): 10 μm. Box-whisker plots shown for stably expressed VcnTS or VcnTS E1015A-E1021A in Vcn−/− MEFs (n = 36, 45 respectively, from N = 3 independent experimental days) quantifying c spread of actin SF orientations (p = 0.34, one-way ANOVA followed by F-test) d SF density per FA-defined local region (p = 0.45, two-tailed Student’s t-test paired with post-hoc Tukey Test), and e angle between FA and average angle of actin filaments within respective Voronoi regions of cells (p = 0.0173, one-way ANOVA followed by F-test). f Schematic depicting transwell setup for directed migration assay. g Box-whisker plots are shown of migrated cells per field of view (FOV) in a Boyden chamber haptotaxis migration assay (n = 19,18,16,18 FOVs per given group from N = 3 independent experiments). Box depicts the median as the center value, the 25th percentile as the lower bound, and the 75th percentile as the upper bound. Whiskers extend 1.5 times the IQR from the bottom and top of box, or to the minimum and maximum of the data if the data does not extend to the whiskers. Values outside the whiskers are plotted as individual points. See Supplementary Table 9 for a detailed listing of exact p values of 8 g. Source data are provided as a Source Data file.
