Fig. 7: CpG islands determine response to CBP/p300 degradation.

a L2FC of RNA Pol2 binding (RRPM) proximal (<=5kB) or distal from the gene TSS at 6 h after dCBP1 treatment (n = 1 independent experiments; Welch’s t-test (p = 0.0) was performed between samples; Box plots of median and quartiles, with whiskers showing 1.5 × inter-quartile ranges). b After 6 h dCBP1 treatment, the motifs of the downregulated peaks indicate their positioning at key transcription factors (TFs) of FP-RMS, MYOD, MYOG, P3F, and SOX8. Additionally, an increased motif enrichment of RNA Pol2 is observed at NFY, a promoter-binding TF that contributes a phenotype similar to that of P3F. NS, not statistically significant. c Percentage of up- and downregulated RNA Pol2 peaks at CpG islands of CBP/p300 independent and dependent genes. <1% of all downregulated RNA Pol2 peaks are located at CpG islands of CBP/p300 dependent gene promoters. d Boxplots showing the Log2(Fold Change) in the Contact Signal (RRPM) of RNA Pol2 loops between DMSO and dCBP1 treated RH4 cells, split by p300 and P3F occupancy and overlap with CpG islands (n = 1 independent experiment; Box plots of median and quartiles, with whiskers showing 1.5 × inter-quartile ranges). e A histogram showing the distribution of RNA Pol2 clusters from RH4 HiChIP based on the overlap between a cluster’s constituent loops and CpG islands. The number of P3F binding sites overlapping the ends of the loops in the cluster is shown in blue.