Fig. 6: The axonal and dendritic landscape of pioneer neurons is altered upon JNK hyperactivation. | Nature Communications

Fig. 6: The axonal and dendritic landscape of pioneer neurons is altered upon JNK hyperactivation.

From: Puckered and JNK signaling in pioneer neurons coordinates the motor activity of the Drosophila embryo

Fig. 6

a and b Flat-prepped, stage 16 embryos expressing mCD8-GFP (a) and mCD8-GFP and HepCA (b) under the control of the RN2-Gal4 line immunostained for GFP. Traces of the aCC (magenta) and RP2 (green) proximal dendrites are over-imposed. Yellow arrowheads point to differences in RP2 axon morphology. Anterior is up. Scale bar is 10 µm. c Histograms showing the mean axonal length ±SD from n = 4 embryos of RN2 controls and n = 4 embryos of RN2/HepCA for the pCC (n = 8 axons for controls and n = 8 axons for experimental), aCC (n = 10 axons for controls and n = 10 axons for experimental) and RP2 (n = 10 axons for controls and n = 9 axons for experimental) neurons. Statistically significant differences in the axonal length of the pCC and RP2 were detected between control and HepCAexpressing neurons. pCC length (****p < 0.0001), aCC length (p = 0.5032) and RP2 length (***p = 0.0006). Control embryos are represented in grey and HepCA expressing embryos in green. d Histograms showing the average axonal wiggliness ±SD from n = 4 embryos of RN2 controls and n = 4 of RN2/HepCA embryos for the pCC (n = 8 axons for controls and n = 8 axons for experimental), aCC (n = 10 axons for controls and n = 10 axons for experimental) and RP2 (n = 8 axons for controls and n = 9 axons for experimental) neurons. RP2 axonal wiggliness was found significantly increased after HepCA expression. pCC wiggliness (p = 0.3662), aCC wiggliness (p = 0.0785) and RP2 wiggliness (***p = 0.0041). e and f Graphs representing the average Dendrite Number (e) and average Dendrite Length (f) of the aCC and RP2 neurons ±SD. For both parameters significant differences were scored in the comparison of control (n = 14) and HepCA (n = 8) expressing neurons. aCC dendrite number (**p = 0.0065), RP2 dendrite number (*p = 0.0266), aCC dendrite length (****p < 0.0001) and RP2 dendrite length (****p < 0.0001). Parametric student t-tests were used to detect statistically significant differences in all cases.

Back to article page