Fig. 3: Loss and Gain of Kcnq2 function in Phox2b neurons differentially affects baseline breathing and central chemoreception. | Nature Communications

Fig. 3: Loss and Gain of Kcnq2 function in Phox2b neurons differentially affects baseline breathing and central chemoreception.

From: Phox2b-expressing neurons contribute to breathing problems in Kcnq2 loss- and gain-of-function encephalopathy models

Fig. 3

A Traces of respiratory activity from Kcnq2+/+ (white), Kcnq2 GOF (red), and Kcnq2 cKO (green) mice during exposure to room air and graded increases in CO2 (0–7%; balance O2). Summary data (n = 15 Kcnq2+/+; n = 9 Kcnq2 GOF; n = 9 Kcnq2 cKO) plotted as mean ± maximum and minimum show respiratory frequency (B; p < 0.0001), tidal volume (C; p = 0.0282) and minute ventilation (D; p < 0.0001) are increased under room air conditions in Kcnq2 cKO mice compared to control or Kcnq2 GOF mice (one-way ANOVA and Tukey’s multiple comparison test). Summary data (Kcnq2+/+ n = 15; Kcnq2 GOF n = 9; Kcnq2 cKO n = 9) plotted as mean ± SEM of frequency (E; p < 0.0001), tidal volume (F; p < 0.0001) and minute ventilation (G; p < 0.0001) show that Kcnq2 GOF mice have a reduced capacity to increase respiratory output in response to graded increases in CO2. Means are compared using two-way ANOVA followed by Tukey’s multiple comparison test and slope of the minute ventilation 0–7% CO2 response is compared using one-way ANCOVA. Asterisk (*) indicates the difference between genotypes; # used to distinguish within genotype differences from control. One symbol = p < 0.05, two symbols = p < 0.01, three symbols = p < 0.001, four symbols = p < 0.0001.

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