Fig. 4: Kcnq2 cKO mice show increased baseline breathing preferentially during the light/inactive state. | Nature Communications

Fig. 4: Kcnq2 cKO mice show increased baseline breathing preferentially during the light/inactive state.

From: Phox2b-expressing neurons contribute to breathing problems in Kcnq2 loss- and gain-of-function encephalopathy models

Fig. 4

A Traces of respiratory activity from Kcnq2+/+ (white) and Kcnq2 cKO (green) mice in room air, 100% O2 and 3–7% CO2 (balance O2) during the light/inactive and dark/active states. Summary data (n = 9 Kcnq2+/+ and n = 12 Kcnq2 cKO mice) plotted as mean ± maximum and minimum frequency (B; p = 0.2180), tidal volume (C; p = 0.0009) and minute ventilation (D; p = 0.0187) shows that Kcnq2 cKO mice exhibit higher baseline respiratory activity during the light/inactive state but not during dark/active conditions. Summary data (Kcnq2+/+ n = 9 and Kcnq2 cKO n = 12 mice) plotted as mean ± SEM of minute ventilation show that Kcnq2+/+and Kcnq2 cKO mice exhibit similar ventilatory responses to CO2 during the light/inactive (E, p = 0.9621) and dark/active states (F, p = 0.0717). Means are compared using two-way ANOVA followed by Tukey’s multiple comparison test and the slope of the minute ventilation 0–7% CO2 response are compared using one-way ANCOVA. Asterisk (*) indicates the difference between genotypes and # designates within genotype differences from control; one symbol = p < 0.05, two symbols = p < 0.01, three symbols = p < 0.001, four symbols = p < 0.0001.

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