Fig. 5: Kcnq2 GOF mice show a blunted ventilatory response to CO2 during the light/inactive state.
A Traces of respiratory activity from Kcnq2+/+ (white) and Kcnq2 GOF (red) mice in room air, 100% O2 and 3–7% CO2 (balance O2) during the light/inactive and dark/active states. Summary data (light/inactive n = 8 mice/genotype; dark/active n = 6 mice/genotype) plotted as mean ± maximum and minimum respiratory frequency (B; p < 0.0001), tidal volume (C; p = 0.0021) and minute ventilation (D; p < 0.0001) show that both genotypes exhibit a characteristic arousal-dependent increase in respiratory activity in the dark/active state and no genotype differences were observed under room air conditions. Summary data (light/inactive n = 8 mice/genotype; dark/active n = 6 mice/genotype) plotted as mean ± SEM of minute ventilation show that CO2/H+-dependent respiratory output in Kcnq2 GOF mice is suppressed in the light/inactive state (E, same data as shown in Fig. 3g, p < 0.0001) but not during the dark/active state where the slope of the ventilatory response to CO2/H+ was similar to control (p = 0.27). Means are compared using two-way ANOVA followed by Tukey’s multiple comparison test and slope of the minute ventilation 0–7% CO2 response are compared using one-way ANCOVA. Asterisk (*) indicates the difference between genotypes and # designates within genotype differences from control; one symbol = p < 0.05, two symbols = p < 0.01, three symbols = p < 0.001, four symbols = p < 0.0001.
