Fig. 5: Functional studies of CAP-TAC1 in feeding and energy balance in mice.
From: A class of secreted mammalian peptides with potential to expand cell-cell communication
a Schematic of the primary amino acid sequence for full-length mouse GDF15 preproprotein and annotation of CAP-GDF15 sequence as well as the canonical GDF15 hormone sequence. b–d Food intake (b), respiratory exchange ratio (RER, c), and ambulatory activity (d) of 12–16-week diet-induced obese male mice following a single treatment of CAP-GDF15 (50 mg/kg, intraperitoneal) or vehicle control. e–g Food intake (e), RER (f), and ambulatory activity (g) of 12–16-week diet-induced obese male mice following a single treatment of scrambled CAP-GDF15 (50 mg/kg, intraperitoneal, scrambled sequence: pGlu-GLEALRARLRV-NH2) or vehicle control. h, i 3-day food intake (h) and body weight (i) of 12–16-week diet-induced obese male mice following treatment with CAP-GDF15 or scrambled CAP-GDF15 (50 mg/kg/day, IP), or vehicle control. Data are shown as means ± SEM. b–d, N = 12 mice/group; for e, f N = 8 mice/group; for h N = 7 food cages for vehicle and CAP-GDF15 groups and N = 5 for the scrambled peptide group; for i N = 14 mice for the vehicle group, N = 13 for the CAP-GDF15 group, and N = 5 for the scrambled peptide group. b–i injection occurred at time T = 0 (5:00 pm) and data were collected for the following 16 hrs. For (b-i), p values were calculated by two-way ANOVA. Source data are provided as a Source Data file.
