Fig. 2: Lifetime retrieval from the numerical model for 4-DASPI.

a Fluorescence lifetime curve for the 4-DASPI sample (the curve has been inverted and normalised so as to appear more familiar compared to standard FLIM curves). Blue shows experimental data (2 s acquisition per delay point) and red depicts a fit from our model described in the Supplementary Material using Equation S11. b The same measurements with a 3.5 s total acquisition time, retrieving the same approximate lifetime. based on fitting to the points shown in green. c Shows the statistical distribution of retrieved lifetimes for N = 260 measurements (3.5 s acquisition time each), indicating a mean of 6.8 ps and a standard deviation of 0.7 ps. d The standard deviation of the lifetime estimation as a function of the total measurement acquisition time. Blue points are the experimental data points and the red line illustrates the expected reduction in uncertainty proportional to the square root of the measurement time.