Fig. 2: Reconstruction of the tissue deformation map for Xenopus hindlimb development.
From: An archetype and scaling of developmental tissue dynamics across species
A Cell labeling by heat shock induction of EGFP expression. Mesenchymal cells around the frontal plane of mid D-V level were irradiated with an infrared laser using the IR-LEGO system. B Integrated data for mesenchymal cell lineages obtained from 11 individuals after morphometric staging and resizing based on mean growth curves. C The workflow for reconstructing the Xenopus limb deformation map (see Methods for details). D, E Spatiotemporal patterns of local tissue deformation for Xenopus (D) and chick (E). The patterns of area growth (top) and deformation anisotropy (bottom) are plotted; the chick pattern is a replot of our previous report with minor modifications14. In the bottom panels, the black line segments indicate the orientation of anisotropy (the length of each segment reflects the magnitude of anisotropy). F The posterior half of the autopod in a later limb bud with a clear skeletal pattern (tXenopus = 54.4 or tChick = 30.5) is derived from the smaller posterior portion of the early limb bud (tXenopus = 50.6 or tChick = 21), clearly showing A-P asymmetric growth; top: chick, bottom: Xenopus. Note that the images of the Xenopus limb bud, except for the top-left photo in panel (A), are inverted in the A-P direction to have the posterior side facing downward. Source data are provided as a Source Data file.
