Fig. 5: Direct activation of α1 adrenergic receptors on OPCs induces Ca2+ transients. | Nature Communications

Fig. 5: Direct activation of α1 adrenergic receptors on OPCs induces Ca2+ transients.

From: Norepinephrine regulates calcium signals and fate of oligodendrocyte precursor cells in the mouse cerebral cortex

Fig. 5: Direct activation of α1 adrenergic receptors on OPCs induces Ca2+ transients.The alternative text for this image may have been generated using AI.

a Pseudocolored average intensity projection of an mGCaMP6s-expressing OPC in an acute brain slice from a NG2-mGC6s;tdT mouse in control (Ctrl) conditions (TTX, 0.5 µM; left) and after bath application of phenylephrine (PE; 10 μM + TTX; 0.5 µM). b CaM map recorded in the Ctrl (left) and after PE application (right). c Intensity vs time Ca2+ traces from 5 CaMs in Ctrl (top) and after PE application (bottom) corresponding to colors in (b). d Heat-map of the intensity and temporal distribution of Ca2+ events in the Ctrl (top) and after PE application (bottom). Graphs showing changes in frequency of Ca2+ events (e), number of active CaMs per minute (f), average amplitude (z-scores) (g), and mean duration of Ca2+ events (h) in Ctrl and after PE application (i) Map of CaMs in control (Ctrl – Glut-B: TTX, 0.5 µM; CNQX, 10 µM; AP5,50 µM; MCPG, 10 µM) (left) and after PE + Glut-B application (right). (j) Intensity vs time Ca2+ traces from 5 CaMs after application of PE + Glut-B corresponding to colors in i (top). Heat-map of the intensity and temporal distribution of Ca2+ events after PE + Glut-B application (bottom). k, l Graphs showing changes in frequency and average amplitude of Ca2+ events in Ctrl and after application of PE + Glut-B. m Map of CaMs in control (Ctrl – Gaba-B: TTX, 0.5 µM; CGP 55845, 5 µM; Gabazine, 5 µM) (left) and after PE + Gaba-B application (right). n Intensity vs time Ca2+ traces from 5 CaMs after PE + Gaba-B application corresponding to colors in m (top). Heat-map of the intensity and temporal distribution of Ca2+ events after PE + Gaba-B application (bottom). o, p Graphs showing changes in frequency and amplitude of Ca2+ events in Ctrl and after application of PE + Gaba-B. All data are presented as mean ± SEM. e–h n = 17 cells; N = 4 mice. e–g Paired t-test: ****P < 0.0001; h Wilcoxon matched-pairs rank test: **P = 0.0011; k, l, o, p n = 24 cells; N = 3 mice; k, l, o Paired t-test: ****P < 0.0001, p Wilcoxon matched-pairs rank test: ****P < 0.0001. Scale bars, 20 µm. Source data are provided in the Source Data file.

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