Fig. 3: Molecular cloning and characterization of MPL1.
From: Control of compound leaf patterning by MULTI-PINNATE LEAF1 (MPL1) in chickpea
a BSA analysis of F2 population derived from mpl1-1 (PI587041) (♀)×WT (cv. ICCV96029) (♂). X-axis shows chromosomes/scaffolds of reference genome, Y-axis is ΔSNP/InDel index between WT and mpl1 pools. Purple and blue lines indicate two-sided 99% and 95% confidence intervals. Black line is mean ΔSNP/InDel index with arrowhead indicating peak above threshold on Chromosome 8. b Enlargement of the peak of the BSA mapping interval. Each triangle represents an annotated gene, with blue indicating genes carrying SNP mutations and red indicating the most likely candidate gene Ca_02268 that simultaneously carried SNP and deletion mutation. c, d Schematic diagram of genomic variations of MPL1 in WT and mpl1 alleles. MPL1 gene, just one exon shown as dark-cyan box, with green and purple boxes representing the C2H2 Zinc-Finger domain and EAR motif, respectively. The mpl1-1 and mpl1-2 alleles had the same mutation (c), consisting of a base substitution (red) and a 14 bp deletion (red dotted lines) within the MPL1 gene, while the mpl1-3 allele carried a 1.5 kb deletion encompassing a portion of the MPL1 gene (d). e Mature compound leaves at the L12 node. f Phylogeny of MPL1 and its homologs from other species, constructed using the maximum-likelihood method and bootstrap test with 2000 replicates. Numbers on nodes represent bootstrap values. MPL1 and its functionally characterized orthologs of Medicago truncatula and Aquilegia coerulea are marked in blue. g Rescued the M. truncatula palm1 mutant phenotype by PALM1pro::MPL1. Shown are representative compound leaves of palm1-5 and an PALM1pro::MPL1 palm1-5 transgenic line. Subcellular localization of 35 S::GFP (h) and 35 S::GFP-MPL1 (i) transiently expressed in tobacco epidermal cells. Three independent experiments were performed with similar results. Scale bars, 1 cm in (e), 0.5 cm in (g), and 10 μm in (h and i).
