Fig. 2: Spatial transcriptome suggests a “cold” TME in AM. | Nature Communications

Fig. 2: Spatial transcriptome suggests a “cold” TME in AM.

From: Delineating the early dissemination mechanisms of acral melanoma by integrating single-cell and spatial transcriptomic analyses

Fig. 2: Spatial transcriptome suggests a “cold” TME in AM.The alternative text for this image may have been generated using AI.

a The first column displays H&E-stained images showing the tumor tissues from five AM patients, with the dotted boxes indicating the selected regions for ST-seq. In the second column, each spot contains an average of 1–10 cells, colored according to CNV levels using Seurat. In the third to sixth columns, spots are colored by the annotated cell types in scRNA-seq data, tumor or non-tumor regions, defined six clusters in tumor regions, and defined five clusters in non-tumor regions using the CARD method. Scale bar, 1000 μm. b Heatmap depicting cluster-specific genes in tumor regions. Source data are provided in the Source Data file. c UMAP plot showing spots in tumor regions, colored based on annotated clusters, CNV levels, and samples. d Heatmap illustrating cluster-specific genes in non-tumor regions. Source data are provided in the Source Data file. e UMAP plot showing spots in non-tumor regions, colored based on annotated clusters, CNV levels, and samples. f Spatial feature plots of annotated cell types from scRNA-seq data in five AM tissue sections with similar results, and representative images were presented. Scale bar, 1000 μm. g Heatmap showing the infiltration levels of annotated cell types in LN-AM (upper panel) and LN+AM (lower panel) patients from tumor to non-tumor regions. Source data are provided in the Source Data file.

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