Fig. 2: Hypomethylation-induced excessive 20E impairs choriogenesis and fertilization. | Nature Communications

Fig. 2: Hypomethylation-induced excessive 20E impairs choriogenesis and fertilization.

From: 5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression

Fig. 2

a PCA of gene expression among four groups: the dsCK day 7 (d7), the dsCK day 9 (d9), the dsDnmt1 d7, and the dsDnmt1 d9. b Volcano plot analysis of the DEGs under dsDnmt1 treatment conditions on d7 and d9. Green dots indicate downregulated genes, and red dots indicate upregulated genes. c Heat map of genes enriched in the pathways of insect hormone biosynthesis. d 20E levels detection under dsDnmt1 treatment on day 9, n = 8 biologically independent samples. e Calculation of hatching rates under exogenous 20E treatment. The atrophied and wizened phenotypes of oothecae in 20E group compared with ethanol control group were showed, n = 3 biologically independent experiments. f DAPI and PHA staining for nuclei and actin protein in ovarian follicle cells. g SEM observation of sponge-like bodies on eggs under exogenous 20E treatment. 8 of 10 sponge-like bodies were observed abnormal. Data are mean ± sd, the differences were analyzed by two-tailed Student’s t test. Source data are provided as a Source Data file.

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