Fig. 1: Characterizing cellular heterogeneity within cell lines by scRNA-seq.
From: Single cell multi-omics reveal intra-cell-line heterogeneity across human cancer cell lines
a Quantitative scRNA-seq analysis of 42 diverse cell lines from 9 lineages. The cell line number of each lineage is indicated. b Schematic of the experimental workflow of scRNA-seq analysis. Three cell lines were pooled for scRNA-seq and then data from Harmonizome was utilized to assign cells to the most similar one based on their gene expression profile. c UMAP plot of all cell lines demonstrating the robustness of cell line assignment. Different cell lines were labeled in different colors. d Graphical representation of single-cell transcriptomics of breast cancer cell lines according to cancer subtype labeled in different colors. e Normalized expression levels of indicated biomarker genes (ESR1 and ERBB2) in individual cells, with redness indicating expression level. f Bubble plot represents the average expression levels of marker genes and fractions of expressed cells in breast cancer cell lines. Basal Epith = Basal Epithelial, Luminal Epith = Luminal Epithelial, L.P. = Luminal Progenitor, EMT = Epithelial to Mesenchymal Transformation. Source data are provided in the Source Data file.
