Fig. 3: Characterizing the heterogeneity of chromatin accessibility within cell lines by scATAC-seq.
From: Single cell multi-omics reveal intra-cell-line heterogeneity across human cancer cell lines
a Schema of the experimental workflow of scATAC-seq analysis. Cell lines were pooled for scATAC-seq and then differential genes were utilized to assign cells to the most similar cell line based on the gene score calculated according to their chromatin accessibility. b UMAP representation of all cell lines’ scATAC profile. Different cell lines were labeled in different colors. c UMAP plot illustration of two types of expression variability: with (differential, MDA-MB-231 and SF268) or without (indiscriminate, COLO 205) distinct chromatin accessibility. d Diversity scores for different cell lines. Cell lines with light blue indicate an indiscriminate pattern and cell lines with red indicate a differential pattern. e A violin plot depicted the diversity score of the cell lines with the two patterns, including differential (n = 15 cell lines) and indiscriminate (n = 25 cell lines). For each boxplot, the center line represents the median, the box indicates the upper and lower quartiles and the whisker represents 1.5-fold of the interquartile range. A one-sided Wilcoxon test was used to test the statistical significance (p-value = 0.045). Source data are provided in the Source Data file.
