Fig. 2: COPII dynamics under nutrient replete conditions. | Nature Communications

Fig. 2: COPII dynamics under nutrient replete conditions.

From: Nutrient deprivation alters the rate of COPII subunit recruitment at ER subdomains to tune secretory protein transport

Fig. 2

a Representative timelapse series showing the distributions of HaloTag fusion proteins following labeling with the JFX650-HaloTag ligand (imaged using lattice light-sheet microscopy). Insets (3x zoom) highlight structures indicated by arrowheads that assemble and disassemble during the imaging window. Bar, 10 μm. b, c Quantification of the average duration of each HaloTag fusion protein (HaloTag-Sec16a, green; HaloTag-Sec23a, light blue; HaloTag-Sec31a, purple; HaloTag-TFG, salmon) at an ER subdomain (b) and the number of new structures that assemble each minute (c). Error bars represent mean +/- SEM (n = 10 cells each; 3 biological replicates; more than 3000 tracked particles each). ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05, as calculated using a one-way ANOVA and Tukey post hoc test. Specific p values are provided in the Source Data file.

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