Fig. 3: Recruitment kinetics of COPII components and associated regulatory factors. | Nature Communications

Fig. 3: Recruitment kinetics of COPII components and associated regulatory factors.

From: Nutrient deprivation alters the rate of COPII subunit recruitment at ER subdomains to tune secretory protein transport

Fig. 3

a Representative timelapse series imaged using lattice light-sheet microscopy showing the accumulation of HaloTag fusion proteins at ER subdomains following labeling with the JFX650-HaloTag ligand. Arrowheads highlight structures undergoing de novo formation in cells. Bar, 1 μm. Quantification of the intensity (b) and instantaneous rate of formation (c) of newly generated sites decorated by HaloTag fusion proteins (HaloTag-Sec16a, green; HaloTag-Sec23a, light blue; HaloTag-Sec31a, purple; HaloTag-TFG, salmon). Error, as displayed by lightly colored bands around smoothed trend lines, represents mean +/- SEM (n = 10 cells each; 3 biological replicates; more than 3000 tracked particles each). Source data are provided as a Source Data file.

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