Fig. 1: Chromatin landscape dynamics in KN9204 and J411.

a Experimental design for generating the epigenomic datasets in KN9204 and J411 under different nitrogen conditions. RNA-seq raw data is from our previous publication¹¹. b Chromatin states are depicted with different colors indicating distinct states: orange for promoter-related, green for transcription-related, pink for enhancer-like, yellow for repressed, and white for no signal. And genomic annotation enrichments were displayed, including emission, coverage, genome annotation, type II TE, and type I TE, alongside gene expression levels associated with each chromatin state. These states can be broadly categorized into five groups: Promoter (Pr), Transcription (Tr), Enhancer-like (En), Repressive (Re), and No Signal (Ns) states. Boxplots show the median, third, and first quartiles. The numbers indicate sample size used in the expression analysis. c Genome browser view of representative genes with dynamic chromatin states under different nitrogen conditions. The color code for the chromatin states are the same as in (b). NN, normal nitrogen; LN, low nitrogen. d The change ratio between different cultivars and nitrogen availabilities for the five categories of chromatin states. The numbers indicate sample size used in the analysis. Boxplots show the median, third and first quartiles. e Variability scores of different histone modifications among diverse tissues, wheat cultivars and nitrogen availabilities. f Cross-correlation heatmap of promoter and distal H3K27ac peaks. Abbreviations were as follow: KN KN9204; J4 J411, NN normal nitrogen, LN low nitrogen, S seed, R root, L flag leaf.