Fig. 3: Mixture of 9-HODE and 13-HODE promoted liver steatosis in mice. | Nature Communications

Fig. 3: Mixture of 9-HODE and 13-HODE promoted liver steatosis in mice.

From: Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity

Fig. 3

Eight-week-old mice were treated with a mixture of 9-HODE and 13-HODE (equal amounts of 9-HODE and 13-HODE and a combined dose of 0.5 μg/g body weight) once a day for 9 days: (a) body weight, liver weight, and liver/body weight; (b) fasting blood glucose level; (c) H&E, Oil Red O, and Nile Red staining of liver sections; scale bar = 100 μm for H&E and Oil Red O; scale bar = 20 μm for Nile Red; (d) liver TG and CHO content; (a–d) n = 9 mice in Ctrl group; n = 7 mice in HODE group; for liver weight and liver/body weight, n = 7 mice per group. e–h RNA sequencing was performed: (e) PCA analysis; (f) heatmap of differentially expressed genes; (g) GO biofunction enrichment analysis of differentially expressed genes; (h) expression level of differentially expressed genes that promote liver steatosis. n = 3 mice per group. i qPCR analysis of the mRNA levels of Fasn, Thrsp, Cd36, Cyp4a14, and Elovl6; n = 9 mice in Ctrl group; n = 7 mice in HODE group. Data represent the mean ± SEM. Two-tailed student’s t test was performed for (a, d) and (Fasn, Thrsp, Cd36 and Cyp4a14 in i); Two-tailed Mann-Whitney test for (b) and (Elovl6 in i). HODEs 9/13-HODEs, TG triglyceride, CHO total cholesterol, FBG fasting blood glucose.

Back to article page