Fig. 2: VEX2 bridges loci on distinct chromosomes.

a–g Super-resolution microscopy analysis of ^GFPVEX2^myc, ^mycVEX2^GFP, ^GFPVEX2/VEX1^myc,VEX2^GFP/VEX1^myc, ^mycVEX2/Pol-I and VEX2^myc/Pol-I. The images are representative, were acquired using a Zeiss Elyra 7 microscope (Lattice SIM²) and correspond to 3D projections by the brightest intensity of 0.1 μm stacks. DNA was stained with DAPI (cyan); scale bars: 2 μm. c The violin plot on the left-hand side indicates the diameter of VEX2 signals when VEX2 is tagged at the N- and/or C-terminus. The black bar corresponds to the median; all datapoints are shown (purple circles). Two-tailed unpaired Student’s t-test; ns, non-significant. The violin plot on the right-hand side indicates the distance between the centroid positions of VEX2 signals. The data are representative of two independent experiments. d–g The graphs represent % of G1 or S phase nuclei (>80 nuclei) where VEX2 N- or C-terminal tag signals overlap, are adjacent or are separate to VEX1 signals (for more details on these assignments, see section ‘Microscopy and image analysis’); averages of two biological replicates and representative of two independent experiments; error bars indicate standard deviation.