Fig. 5: The N-terminal fragment of VEX1 interacts with VEX2. | Nature Communications

Fig. 5: The N-terminal fragment of VEX1 interacts with VEX2.

From: An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome

Fig. 5

a Representative images of VEX1-Nmyc (endogenous level expression) colocalising with GFPVEX2. The graph shows the relationship between VEX1 and VEX2 signals in G1 nuclei and shows averages of two biological replicates that are representative of two independent experiments; error bars correspond to standard deviation. The images were acquired using a Zeiss LSM880 Airyscan (>100 nuclei quantified) and correspond to maximum-intensity 3D projections of 0.1 μm stacks; DNA was stained with DAPI (cyan); scale bar: 2 μm. b Co-immunoprecipitation of GFPVEX2/VEX1myc, GFPVEX2/VEX1-Nmyc and GFPVEX2/VEX1-Cmyc with GFP-Trap magnetic agarose beads (Chromotek) followed by protein-blot analysis; lysis was in HEPES/NaCl buffer and blotting was with α-VEX2 and α-myc. Green, GFP; magenta, myc; I-input; E-elution. All proteins were endogenously tagged, except for the C-terminal region of VEX1, which was overexpressed (24 h induction). The protein blots are representative of two independent experiments. c Summary of VEX1 and VEX2-associated features and phenotypes. NLS nuclear localisation signal.

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