Fig. 8: Distinct patterns of VSG derepression in VEX1 or VEX2-depleted cells. | Nature Communications

Fig. 8: Distinct patterns of VSG derepression in VEX1 or VEX2-depleted cells.

From: An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome

Fig. 8

a, b Low dimensional (UMAP) plots of single cells following VEX1 knockdown (after filtering). Each point is the VSGome (sum of the VSG transcripts in the transcriptome) of one cell positioned according to similarity with neighbouring VSGomes, coloured by number of VSGs expressed per cell (a) or expression of specific VSG combinations (b). The pie chart in (a) shows the % of cells expressing 1–5 VSG transcripts per cell following VEX1 depletion. c, d Low dimensional (UMAP) plots of each cell following VEX1 or VEX2 knockdown (after filtering). c Cells are coloured based on their origin: VEX1 knockdown dataset (orange) or VEX2 knockdown dataset (blue). d Cells are coloured based on the number of VSGs expressed per cell. e The histogram represents the % of cells expressing 1 to >9 VSG transcripts per cell following VEX1 (cyan) or VEX2 (salmon) knockdown.

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