Table 1 Eleven different fluid combinations in the dataset make it possible to investigate the effects of fluid properties on droplet generation

From: Design automation of microfluidic single and double emulsion droplets with machine learning

	Dispersed fluid		Continuous fluid		Interaction
	Fluid	Viscosity (mPa ⋅ s)	Fluid	Viscosity (mPa ⋅ s)	Interfacial tension (mN/m)	Device material
	Fluids included in the comprehensive dataset
1.	M9 bacterial media	0.861	HFE 7500 oil + 2.2% ionic Krytox	1.61	12.84	PDMS
2.	M9 bacterial media + 25 mM glucose	0.967	HFE 7500 oil + 2.2% ionic Krytox	1.61	11.60	PDMS
3.	PBS	0.931	HFE 7500 oil + 2.2% ionic Krytox	1.61	0.543	PDMS
4.	PBS + 1% Tween-20	0.988	HFE 7500 oil + 2.2% ionic Krytox	1.61	0.319	PDMS
5.	PBS + 0.9% NP40	1.003	HFE 7500 oil + 2.2% ionic Krytox	1.61	1.41	PDMS
6.	PBS + 10% PEG 6000 mw	3.431	HFE 7500 oil + 2.2% ionic Krytox	1.61	0.461	PDMS
7.	DI water	1.001	NF350 mineral oil + 5% Span 80	57.2	5.0	PC^a
8.	HFE 7500 oil + 2.2% ionic Krytox	1.61	PBS+1% Tween-20 + 2% Pluronic F68	1.303	0.318	PDMS^b
9.	HFE 7500 oil + 2.2% ionic Krytox	1.61	M9 salts + 2% Pluronic F68	1.412	0.522	PDMS^b
10.	HFE 7500 oil + 2.2% ionic Krytox	1.61	M9 salts + 25 mM glucose + 2% Pluronic F68	1.563	0.458	PDMS^b
11.	HFE 7500 oil + 2.2% ionic Krytox	1.61	PBS + 10% PEG 6000 mw + 2% Pluronic F68	6.395	0.455	PDMS^b
	Unseen fluids for assessing the generalizability of models
12.	DMEM complete cell media + 16% Optiprep	1.25	2% dSurf HFE 7500 oil	1.61	9.74	PDMS
13.	RPMI 1640 complete cell media + 20% Optiprep + 0.1% Pluronic F127	1.36	2% dSurf HFE 7500 oil	1.61	6.16	PDMS
14.	2% dSurf HFE 7500 oil	1.61	RPMI 1640 complete cell media + 5% Pluronic F127	2.82	4.61	PDMS^b
15.	Surfactant-free HFE 7500 oil	1.31	Trimethylolpropane trimethacrylate (TMPTMA)	42	3.10	PDMS^b
16.	Trimethylolpropane trimethacrylate (TMPTMA)	42	50% glycerol in water	6	15.3	PDMS^b
17.	52% w/w glycerol in water (surfactant-free)	7	Silicone oil	4.6	29	Glass
18.	52% glycerol in water + 50 mM dodecyltrimethylammonium bromide	7	Silicone oil	4.6	10	Glass
19.	52% glycerol in water + 5 mM hexadecyltrimethylammonium bromide	7	Silicone oil	4.6	7.3	Glass

These fluids are commonly used across different life science applications. The viscosity of the dispersed fluids varied from 0.86 to 3.4 mPa ⋅ s and the viscosity of the continuous fluids varied from 1.61 to 57.2 mPa ⋅ s. The interfacial tension between the dispersed and continuous fluids ranged from 0.318 to 12.84 mN/m. In addition, generated data and previously published data on droplet generation using 8 unseen fluid combinations were used to assess the generalizability of models to unseen fluids.
^aPolycarbonate.
^bPDMS made hydrophilic with plasma treatment.

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Table 1 Eleven different fluid combinations in the dataset make it possible to investigate the effects of fluid properties on droplet generation

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