Fig. 3: TOP2B is phosphorylated by ERK1 and ERK2 proteins.
From: ERK2-topoisomerase II regulatory axis is important for gene activation in immediate early genes
a Immobilized template assay results presenting TOP2B enrichment by ERK1m and ERK2 on the WT template but not on the ELK1mut template (n = 4 independent experiments). Data are presented as mean values and SD. P values for the bar graphs were calculated with the unpaired, one sided Student’s t test. b Deubiquitinated TOP2B used for the in vitro kinase assays, silver-stained. c Silver-stained reactions following the in vitro kinase assay. d Autoradiograms of the in vitro kinase assay with TOP2B and ERKs. ELK1, a positive control. e In vitro kinase assay followed by SDS-PAGE for the preparation of mass spectrometry analyses. Red boxes showing sliced gel pieces used for the analyses. CTRL, ERK storage buffer only; SM, size marker (KDa). f A flow chart showing the steps of mass spectrometry analyses performed in this study. g A schematic representation of the TOP2B residues phosphorylated by ERK2/ERK2m (red), or both ERK1/ERK1m and ERK2/ERK2m kinases (blue). Source data are provided as a Source Data file.
