Fig. 6: ERK2 function is required for normal TOP2B behavior and transcriptional activation.
From: ERK2-topoisomerase II regulatory axis is important for gene activation in immediate early genes
a qRT-PCR results presenting the effects of ERK2 catalytic inhibition on EGR1 and HSP70 transcription. HEK293 cells were not synchronized (n = 3). Data are presented as mean values and SD. P values for the bar graphs were calculated with the unpaired, one sided Student’s t test. b qRT-PCR results presenting the effects of ERK2 catalytic inhibition on the EGR1 and FOS transcription (n = 3). HEK293 cells were synchronized at G0 (S0) before they were serum-induced to early G1 (S15). Data are presented as mean values and SD. P values for the bar graphs were calculated with the unpaired, one sided Student’s t test. c ChIP-qPCR results showing S2 Pol II occupancy changes in the EGR1 gene with or without functional ERK2 (n = 3 independent experiments). Data are presented as mean values and SD. P values for the bar graphs were calculated with the unpaired, one sided Student’s t test. d ChIP-qPCR results showing a dramatic increase of TOP2B at EGR1 upon the catalytic inhibition of ERK2 (n = 3 independent experiments). Data are presented as mean values and SD. P values for the bar graphs were calculated with the unpaired, one sided Student’s t test. e Immunoblots of ERK1 (K1KD), ERK2 (K2KD), and ERK1/ERK2 double KD (DoubleKD) HEK293 cells. SCR, scrambled siRNA; Tubulin, a reference. f qRT-PCR and ChIP-qPCR results presenting the effects of ERK KDs on EGR1 transcription (left) and TOP2B occupancies at the EGR1 TSS (right), respectively (n = 3 independent experiments). Data are presented as mean values and SD. P values for the bar graphs were calculated with the unpaired, one sided Student’s t test. g qRT-PCR and ChIP-qPCR results presenting the effects of ERK KDs on FOS transcription (left) and TOP2B occupancies at the FOS TSS (right), respectively (n = independent experiments). Data are presented as mean values and SD. P values for the bar graphs were calculated with the unpaired, one sided Student’s t test. Source data are provided as a Source Data file.
