Fig. 7: RBC from chronically hyperglycemic mice and patients with diabetes mellitus type 2 have higher S1P and PP2A  and lower GLUTs and glucose uptake. | Nature Communications

Fig. 7: RBC from chronically hyperglycemic mice and patients with diabetes mellitus type 2 have higher S1P and PP2A  and lower GLUTs and glucose uptake.

From: Sphingosine-1-phosphate suppresses GLUT activity through PP2A and counteracts hyperglycemia in diabetic red blood cells

Fig. 7

C57Bl6 mice were fed either standard chow diet or high caloric diet (DIO diet) for 12 weeks and the following parameters were determined. A Fasting plasma blood glucose levels (n = 5/6), (B) HbA1c (n = 8/8), (C) S1P concentrations (n = 11/12), (D) PP2A activity (n = 8/7), (E) glucose uptake (n = 8 each), and (F) Sphk activity (n = 4 each) based on the conversion of C17-sphingosine to C17-S1P as measured by LC-MS/MS (n = 4). G HbA1c levels of human patients with and without T2DM (n = 13 case-controls, characteristics in Supplementary Table 1). H Intracellular S1P concentrations (n = 13 each), (I) PP2A activity (n = 10/9) and (J) cell surface GLUT1 (n = 8/6) on RBC from patients with and without T2DM (randomly selected from above groups). K Correlation between intracellular S1P and PP2A activity in RBC from T2DM patients (n = 10). Data are presented as mean ± sd and tested with two-tailed unpaid t test (A–I) and two tailed Pearson correlation coefficient (J); p* < 0.05; p**<0.01; p**** < 0.0001.

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