Fig. 2: MlaC interacts with MlaA in a manner that juxtaposes the MlaA channel and the MlaC lipid binding cavity.
From: Molecular mechanism of phospholipid transport at the bacterial outer membrane interface
a, b Representative immunoblots showing formation of disulfide crosslinks between MlaA and MlaC in ΔmlaA ΔmlaC cells expressing cysteine-substituted MlaA-His and MlaC-His from the pCDF and pET22/42 plasmids, respectively. Samples were subjected to non-reducing (top) or reducing (bottom) SDS-PAGE prior to immunoblotting. The experiment had been performed at least three times with similar results. Source data are provided as a Source Data file. c Cartoon representation of KpMlaA (cyan) (PDB: 5NUP)32 and holo-MlaC (pink, PL in red spheres) (PDB: 5UWA)23. Residues on MlaC which displayed disulfide crosslinking with MlaA, and vice versa, are illustrated in red and blue sticks, respectively. MlaC residues mapped to MlaA residues Q205C, M212C and F223C are connected by beige, blue and pink lines respectively.
