Fig. 1: The CROPs domain protects TcsL from autoproteolysis.
a A Schematic drawing of the TcsL truncations designed for this study. b Purified full-length TcsL and TcsL1–1808 were separated on a Coomassie-stained SDS-PAGE gel. TcsL1–1808 was largely fractured, leaving a ~ 140 kDa fragment (light dotted box). c The large fragment in (b) was analyzed by mass spectrometry. The black arrows indicate a breaking point between Leu543 and Gly544. d Purified TcsL1–1808 and TcsL1-1808/C698S were separated on a Coomassie-stained SDS-PAGE gel. e Purified TcsL, TcsL1–2260, TcsL1–1977, TcsL1–1921, TcsL1–1893, and TcsL1–1808 were separated on a coomassie-stained SDS-PAGE gel, only TcsL1–1808 showed overt autoproteolysis. f The autocleavage of TcsL, TcsL1–2260, TcsL1–1921, and TcsL1–1893 was induced by the gradient concentrations of InsP6 for 3 hours. The percentage of cleavage was measured and plotted on the chart. g Purified full-length TcsL, TcsL∆1848-1869, and TcsL∆1848-1869/C698S were separated on a coomassie-stained SDS-PAGE gel. Source data are provided as a Source Data file.
