Fig. 3: SIRT6 deficiency protects against airway remodeling in mouse model of asthma.
From: Epithelial SIRT6 governs IL-17A pathogenicity and drives allergic airway inflammation and remodeling
a Schematic illustrating the genetic approach used to generate airway epithelium cell–conditional knockout of Sirt6 (AE-Sirt6Δ/Δ) mice. b SIRT6 deficiency was confirmed by assessing genomic DNA. c–f Representative periodic acid-Schiff (PAS) staining and Masson of lung sections from AE-Sirt6fl/fl and AE-Sirt6Δ/Δ mice treated with HDM/LPS in a chronic severe asthma (CSA) model and their quantification (n = 5 per group per study). Scale bars, 100 μm. g Western blot analysis of the EMT markers N-Ca, vimentin or the myofibroblast markers α-SMA and Collagen I in lung homogenate from AE-Sirt6fl/fl and AE-Sirt6Δ/Δ mice treated with HDM/LPS in CSA model (n = 5 per group per study). h, i qRT-PCR analysis of the EMT regulators Fn1 and Snai1 in lung homogenate from AE-Sirt6fl/fl and AE-Sirt6Δ/Δ mice treated with HDM/LPS in CSA model (n = 5 per group per study). j–m Total BAL fluid (BALF) cells, differential cell counts, and histologic analysis of the lung sections were performed with hematoxylin and eosin staining to visualize inflammatory cell recruitment from AE-Sirt6fl/fl and AE-Sirt6Δ/Δ mice treated with HDM/LPS in ASA model (AE-Sirt6fl/fl-Control, AE-Sirt6Δ/Δ-Control, AE-Sirt6fl/fl-Asthma n = 6; AE-Sirt6Δ/Δ-Asthma n = 7). Data are shown as means ± SEM and three or more independent experiments were performed. Significance was calculated by one-way ANOVA followed by Tukey’s post-hoc test for (d–f–h–k–m).
