Fig. 1: Coacervation of Fe(CN)63−/ Fe(CN)64− and polypeptides.
From: Selective amide bond formation in redox-active coacervate protocells
a Schematic illustration of associative liquid-liquid phase separation of Fe(CN)63−/Fe(CN)64− and pLys to produce coacervate droplets. b Optical microscope images of Fe(CN)64−/pLys droplets prepared at 1 mM Fe(CN)64− and 5 mM pLys (monomer basis), and (c) at 2 mM Fe(CN)63− and 5 mM pLys (monomer basis). Insets show photographs of the corresponding turbid suspensions. Scale bars, 10 μm. Fluorescence microscopy images of Fe(CN)64−/pLys droplets with various client molecules: (d) pyranine, (e) NADPH, (f) poly-rU15. Scale bars correspond to 10 μm. g Critical salt concentration of Fe(CN)63− and Fe(CN)64− coacervates with a fixed concentration of 5 mM pLys, determined from turbidity titrations. Shaded regions indicate conditions under which coacervate microdroplets were observable. Solid lines are guides to the eye. The experiments in b–f were repeated three times with similar results. Each point in g represents an independently prepared sample.
