Fig. 3: Peptide bond formation in Fe(CN)63−-based droplets.
From: Selective amide bond formation in redox-active coacervate protocells
a Schematic illustration of Fe(CN)63−-based droplets as microreactors for peptide ligation in water. b 1H NMR spectrum showing the peptide ligation reaction of N-acetyl-glycine thioacid Ac-Gly-SH (8 mM, green) and Gly-OH (3 eq., blue) with Fe(CN)63−/pLys(Me)3 (gray) coacervates (1 eq., pH 9, room temperature) to yield Ac-Gly-Gly-OH (orange). c Plot of % ligation products versus time for peptide ligation reaction in dilute phase and Fe(CN)63−-based coacervate droplets. Each time point represents an independently prepared sample. Symbols: samples represented by circles, diamonds and triangles were prepared and analyzed together as a series; samples represented by squares, hexagons and inverted triangles were separately prepared and analyzed as a series. Different symbols correspond to yields determined from different peaks. d Plot of selective peptide bond formation within coacervate droplets. Each time point represents an independently prepared sample.
