Fig. 4: T cell subtypes analysis. | Nature Communications

Fig. 4: T cell subtypes analysis.

From: Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment

Fig. 4

a UMAP plot of immune cells subtypes (n = 10,672 cells). Re-clustering of CD8 + T cells (left, n = 2382 cells) and CD4 + T cells (right, n = 2840 cells). The number of samples is 4. b Heatmap of marker genes expressed in CD8+ and CD4 + T cells. The color above the heatmap corresponds to the clusters in UMAP show in (a). c The cell ratio of all CD8+ and CD4 + T cells. Source data are provided as a Source data file. d Trajectories of CD8+ (upper) and CD4+ (below) T cells from pseudotime analysis. e Spatial distribution of tumor cells, partial subgroups of CD8 + T cells and CD4 + T cells. f IHC staining validated the expression of CD3 and PDCD1 in the tumor regions and non-tumor regions in four seminoma samples. The bar plot represented the cell ratio of PDCD1+ cells (dysfunctional cells). The white color represents the PDCD1+/CD3+ cell ratio in non-tumor region and the black color represents the PDCD1+/CD3+ cell ratio in tumor region. P value was calculated by two-sided Wilcoxon rank-sum test. *P < 0.05. Data are presented as “mean values ±SEM” as appropriate. Source data are provided as a Source data file. Scale bar, 50 μm. g Survival analysis showing that FOXP3 and IL21 high expression was associated with worse overall survival (OS) and disease-specific survival in the TCGA cohorts.

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