Fig. 5: MMP9 and CTSK facilitated seminoma to break through the tubules. | Nature Communications

Fig. 5: MMP9 and CTSK facilitated seminoma to break through the tubules.

From: Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment

Fig. 5

a Diagram of tumor cells breaking through the seminiferous tubules. This plot created with BioRender.com. b Expression of selected pathway in spatial transcriptome data of three seminoma samples. c scRNA-seq data showed that MMP9 mainly expressed in macrophage. d Correlation analysis of MMP9 and CTSK expression with macrophage location in spatial transcriptomics data using Pearson’s correlation coefficients. Points represent the cells in macrophage. Exact pvalue of MMP9 and macrophage: 2.08E–296; Exact pvalue of CTSK and macrophage: 5.01E–32. Source data are provided as a Source data file. e MMP9 and CTSK expression patterns in immune cells of scATAC-seq data from Fig. 3b. CD68 and LYZ: macrophage markers. f Survival analysis showing that MMP9 and CTSK high expression was associated with worse overall survival (OS) and Disease-specific survival in the TCGA cohorts. g IF staining of MMP9 and CTSK in tissue sections of seminoma patients (n = 3). White arrow represents positive signal. The barplot showed the percentage of MMP9+/CTSK+ cells in CD68+/CD68− cells. The height of the bar represents the cell ratio of MMP9+/CTSK+ cells in CD68+/CD68- cells. P value was calculated by one tailed paired t test. **P < 0.01. Data are presented as “mean values ± SEM” as appropriate. Source data are provided as a Source data file. Scale bar, 50 μm and 20 μm in magnified regions.

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