Table 1 SNT50 and SNT90 values determined by single-virus or multiplex-virus assays for antisera against DENV-2, JEV or YFV

From: Multiplexed multicolor antiviral assay amenable for high-throughput research

  

Anti-DENV-2 Serum

Anti-JEV Serum

Anti-YFV Serum

Infection

Virus

SNT50

SNT90

SNT50

SNT90

SNT50

SNT90

Single-virus infection

DENV-2/mAzurite

725 ± 136

184 ± 23

65 ± 4

n.d.

199 ± 97

n.d.

 

JEV/eGFP

n.d.

n.d.

4885 ± 914

732 ± 75

n.d.

n.d.

 

YF17D/mCherry

n.d.

n.d.

n.d.

n.d.

12759 ± 6468

1888 ± 157

Multi-virus infectiona

DENV-2/mAzurite

1370 ± 70

168 ± 55

49b

n.d.

n.d.

n.d.

 

JEV/eGFP

n.d.

n.d.

4354 ± 195

1121 ± 21

n.d.

n.d.

 

YF17D/mCherry

n.d.

n.d.

n.d.

n.d.

14444 ± 4648

2849 ± 393

  1. Values shown as mean ± SD.
  2. n.d. non-detectable.
  3. aMulti-infection: Infection of DENV-2/mAzurite, JEV/eGFP and YF17D/mCherry.
  4. bOnly two times out of the three individual experiment reach 50% of the neutralization, with as calculated SNT50 1:49.
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