Table 2 EC50 and EC90 values for interferon alpha (IFN-α), the pan-flavivirus polymerase inhibitor NITD008, and the DENV-specific inhibitor JNJ-A07 as determined in the single-virus or multiplex-virus assays

From: Multiplexed multicolor antiviral assay amenable for high-throughput research

  

IFN-α (IU/mL)

NITD008 (μM)

JNJ-A07 (nM)

Infection

Virus

EC50a

EC90a

EC50

EC90

EC50

EC90

Single-virus infection

DENV-2/mAzurite

8.6 ± 1.9

156 ± 24

1.5 ± 0.2

6.1 ± 1.3

0.2 ± 0.1

0.7 ± 0.2

 

JEV/eGFP

1.9 ± 0.3

10 ± 1.1

0.9 ± 0.1

3.6 ± 0.4

n.i.

n.i.

 

YF17D/mCherry

1.6 ± 0.1

9.6 ± 0.3

0.9 ± 0.1

2.7 ± 0.3

n.i.

n.i.

Multi-virus infectionb

DENV-2/mAzurite

42 ± 9.0

457 ± 258

2.6 ± 0.7

7.2 ± 0.8

0.2 ± 0.02

0.7 ± 0.1

 

JEV/eGFP

1.8 ± 0.3

9.1 ± 0.5

1.1 ± 0.4

3.7 ± 0.4

n.i.

n.i.

 

YF17D/mCherry

1.9 ± 0.2

11 ± 1.7

0.7 ± 0.2

2.2 ± 0.3

n.i.

n.i.

  1. Values shown as mean ± SD.
  2. n.i. no inhibition.
  3. aExtrapolated values in case lowest concentration of IFN-α used still resulted in some virus inhibition (infected cell count <100% of untreated virus control).
  4. bMulti-infection: Infection of DENV-2/mAzurite, JEV/eGFP and YF17D/mCherry.
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