Fig. 6: SERK3 is required for LcPIP1-induced cell death.

a Cell death triggered by LcPIP1 in wild-type (WT) and BAK1-knockout lines. N. benthamiana leaves were infiltrated with Agrobacterium tumefaciens strains carrying C-terminal HA-tagged LcPIP1. Photographs were taken at 4 dpa. b LcPIP1-HA, INF1-RFP, and RFP (as a vector control for INF1-RFP) were expressed in both WT and bak1 plants. Quantification of cell death by measuring electrolyte leakage at 2 dpa. Electrolyte leakage from infiltrated leaf discs was measured as a percentage of leakage from boiled discs. Data are shown as the mean ± SE (n = 8–10 biologically independent samples). Different letters on the graph represent significant differences among samples (One-way ANOVA; P < 0.05). c Protein expression of LcPIP1-HA, INF1-RFP, and RFP was verified by western blot. Red asterisks indicated protein bands of the correct size. Ponceau S staining of Rubisco was used to indicate loading quantity of protein samples. d, e The GFP- or LcPIP1- expressing WT and BAK1-knockout lines, were inoculated with mycelia plugs of Ph. capsici, and the lesion diameters were measured at 48 hpi. Data are shown as the mean ± SE (n = 13–16 biologically independent samples). Two-tailed Student’s t-test was used for significance analysis. f DNA from Ph. capsici infected regions was isolated and relative Ph. capsici biomass was measured to evaluate the severity of infection by qPCR. Data are shown as the mean ± SE of six replicates. Two-tailed Student’s t-test was used for significance analysis. These experiments were repeated three times with similar results. Source data are provided as a Source Data file.