Fig. 4: Effects of TFCP2 fusions on myogenic differentiation.
a Stages of muscle cell differentiation from myoblasts to myotubes and corresponding expression pattern of MYOD and MYOG transcription factors and myosin heavy chain (MHC). The image was created with BioRender.com and adapted from Bentzinger et al.64. b Immunofluorescence images of LHCN-M2 cells transduced with EV or FUS-TFCP2 and cultured in differentiation medium for six days. Green, MyoHC; blue, DAPI (nuclei). Representative images of three independent experiments with similar results are shown. Scale bar, 1 mm. c Fusion index of LHCN-M2 cells transduced with EV or FUS-TFCP2 and cultured in differentiation medium for five days. Mean ± SEM (n = 3 independent experiments). d Relative MYOD and MYOG mRNA expression of LHCN-M2 cells transduced with EV or FUS-TFCP2 and cultured in differentiation medium for six days. Mean ± SEM (n = 3 independent experiments). e Phase-contrast images of LHCN-M2 cells transduced with EV, FUS, TFCP2, or FUS-TFCP2 and cultured in differentiation medium for ten days. Representative images of six independent experiments with similar results are shown. Scale bar, 1 mm. Source data for c and d are provided in the Source Data file.
