Fig. 2: Transmembrane domain (TMD) of ELIC in different nanodiscs.
From: Lipid nanodisc scaffold and size alter the structure of a pentameric ligand-gated ion channel
a Pore profile of apo-spMSP1D1ELIC (light blue) and MSP1E3D1ELIC5 (deep purple) showing constriction at 16’ and 9’ in the apo structure. b Top view of a TMD subunit of apo-spMSP1D1ELIC and MSP1E3D1ELIC5 with red and blue boxes indicating changes in M2 (c) and M4 and (d), respectively. c Side view of M2 with zoomed-in views of the top and bottom of the helix, and a plot of the M2 Cα-atom distance to the pore axis for SMAELIC (lime green), MSP1E3D1ELIC (cyan), spNW15ELIC (orange), and MSP1E3D1ELIC5 (deep purple). For clarity, MSP1E3D1ELIC is represented as cyan with black triangles in the M2 Cα-atom distance plot. d Top views of M4, and graph of the M4 tilt angle relative to the pore axis for apo-MSP1E3D1ELIC (raspberry), apo-spMSP1D1ELIC (light blue), SMAELIC (lime green), saposinELIC (deep olive), MSP1E3D1ELIC (cyan), spMSP1D1ELIC (gray), MSP1E3D1ELIC5 (deep purple), and spNW15ELIC5 (pale yellow). All images are from a superposition of the TMD.
