Fig. 7: Cefoperazone/sulbactam reduces mast cell activation in Klebsiella pneumoniae-exacerbated PCA reaction.
From: Gut microbiota facilitate chronic spontaneous urticaria
a Experimental design of cefoperazone/sulbactam treated Klebsiella pneumoniae transplanted mice. b Representative ear images and the quantification of Evans blue dye. Control (n = 4) vs KPn_PCA (n = 7), P < 0.0001; KPn_PCA vs KPn_CPZ/SBT_PCA (n = 7), P = 0.0006. c Representative images of toluidine blue (×400 magnification) and the percentages of degranulated MCs in ear. Control (n = 3) vs KPn_PCA (n = 4), P = 0.0021; KPn_PCA vs KPn_CPZ/SBT_PCA (n = 4), P = 0.0164. d mRNA expression of Il4, Il13, Tnfα and Il10 in mouse ear skin. Control vs KPn_PCA, P = 0.0034 (Il4), 0.0003 (Il13), 0.0003 (Tnfα), and 0.0185 (Il10), respectively; KPn_PCA vs KPn_CPZ/SBT_PCA, P = 0.0037 (Il4), 0.0002 (Il13), 0.0064 (Tnfα), and 0.0080 (Il10), respectively. e Plasma level of LPS detected by ELISA. Control (n = 4) vs KPn_PCA (n = 6), P = 0.0003; KPn_PCA vs KPn_CPZ/SBT_PCA (n = 6), P = 0.0010. f Schematic of gut microbiota facilitating chronic spontaneous urticaria. One-way ANOVAs with Tukey’s multiple comparisons test was used for b right, c right, d and e. The data are presented as mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. CPZ/SBT cefoperazone/sulbactam. The number of biologically independent samples used in d is depicted in the figure. Source data are provided as a Source Data file.
