Fig. 6: SOX9 regulates SRSF5 splicing in human beta cells.
From: Sox9 regulates alternative splicing and pancreatic beta cell function
A Single cell sequencing of iSOX9KO cells with or without DOX from day 12 till day 28. B Populations identified from the single cell data. Markers used to annotate the clusters are shown. C Overlapping iSOX9KO eBC populations with and without DOX. D Fraction of cells populating different clusters with or without DOX addition. E SRSF5 transcript compatibility counts (TCCs) for control (grey) and DOX-treated (red) cells. Bars correspond to the mean TCCs for Dox (n = 2392) and for No Dox (n = 2660) cells in cluster 3. Error bars correspond to 95% confidence intervals around the mean estimated by bootstrapping (n = 1000). F Strategy to knockout SOX9 and inhibit nonsense-mediated degradation in iSOX9KO eBCs and test for isoform switching in SRSF5 by PCR. No DOX Veh, grey bar; No DOX CHX, orange bar; DOX Veh, blue bar; DOX CHX, cyan bar. n = 5 per group. Error bars represent S.E.M. using a two-tailed Student’s t test, *p < 0.05. Source data are provided as a Source Data file.
