Fig. 1: Matrisome dynamics during aging.

a, d, g aging time course of matrisome mRNA levels were classified as down- (a), unchanged (d), or up-regulated (g) during aging based on the agreement between the Pearson and Spearman correlation coefficients when applied to the individual samples. (Source data: GSE46051, Supplementary Data 2). b, e, h Quantitative whole proteome analysis aging time course matrisome protein levels declining (b), unchanged (e), or increasing (h) during aging, taking into account the aging correlation of the individual samples (Data and statistics Supplementary Data 3). c, f, i Classification of the de novo protein synthesis rate of each protein into lower (c), unchanged (f), or elevated (i) during aging based on the difference between the synthesis rate of the individual protein to the overall mean protein production. (Source data: PMID: 25686393, Supplementary Data 3). j–o The aging time course of collagens that are incorporated into the ECM. j, l, m Model extrapolated from transgenic fluorophore tagged collagens shown as representative images of one independent biological trial (k, n, o). Note in k, the strong yellow-brownish fluorescence is autofluorescence from gut granules (see Methods for details). COL-120::GFP is in green localized in the cuticular ECM. Details in Supplementary Fig. 1, Supplementary Data 1. Scale bar = 50 µm.