Fig. 2: Characterisation of Ir-NP and its ratiometric imaging. | Nature Communications

Fig. 2: Characterisation of Ir-NP and its ratiometric imaging.

From: A light-activatable theranostic combination for ratiometric hypoxia imaging and oxygen-deprived drug activity enhancement

Fig. 2

a The hydrodynamic size distribution of Ir-NP. b The TEM image of Ir-NP. Scale bar is 200 nm. (n = 3 independent experiments). c The UV-vis absorption of Ir-NP in aqueous solutions. d The emission of Ir-NP with different oxygen levels in aqueous solutions, λex = 620 nm. e The linear correlation of the ratio (I774 / I665) response to oxygen levels of Fig. 1d. f Reversible ratio of Ir-NP in nitrogen- and oxygen-saturated aqueous solution. g Luminescence imaging and ratio-metric results of Ir-NP with different concentration under different oxygen concentrations. Ratio = Ch1 (675 ± 25 nm)/Ch2 (775 ± 25 nm). h The linear correlation of the ratio response to oxygen levels of Fig. 1g. The statistical data are expressed as mean values  ±  S.D. (n = 7 independent experiments). The term (arb. units) is abbreviated for arbitrary units.

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