Fig. 3: Knockout of ATH leads to embryo lethality. | Nature Communications

Fig. 3: Knockout of ATH leads to embryo lethality.

From: Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage

Fig. 3

a Seed development in the siliques of wild-type and ath+/− plants at 10 days (upper panel) and 15 days (lower panel) after flowering (DAF). The asterisks (*) indicate the aborted seeds. Scale bar, 1 mm. b Percentage of aborted seeds in the siliques shown in (a). Data were calculated from 10 siliques for each genotype, indicated by individual dots. The graphs represent the mean ± SD. ****P < 0.0001 by unpaired two-sided t test. c Genotyping of wild-type and lethal seeds in the siliques shown in (a). Experiments were repeated three times with similar results. d Embryo development in wild-type and aborted seeds of the siliques of ath+/− plants. DAF, days after flowering. The wild-type embryos showed normal developmental processes: globular-shaped (4 DAF), heart-shaped (6 DAF), torpedo-shaped (7 DAF), and cotyledon-shaped (8 DAF). Scale bar, 100 μm. Experiments were repeated three times with similar results. Source data are provided as a Source Data file.

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