Fig. 3: Changes in 6mer seed viability of R-sRNAs with age.

a 6mer seed viability graph of differentially expressed R-sRNAs of 2- (2 M) and 18- (18 M) month-old mouse brains. Shown are averages of triplicate samples. sRNAs that have a difference in expression of 1000 or more reads are labeled. Enriched sRNAs are shown in green and depleted sRNAs in red. Insert: 6mer seed viability plot of the differential 6mer seed viability of the analysis. b 6mer seed viability graph of total R-sRNAs of iPSC-derived neurons aged for 1 and 5 months (one sample each). sRNAs with 10,000 or more reads are labeled. In each label sRNAs are listed in the order of expression levels. Enriched sRNAs are shown in green and depleted sRNAs in red. Insert: 6mer seed viability plot of the differential 6mer seed viability of the analysis. Western blot insert: Analysis of iPSC-derived neurons aged in vitro for 30, 150 or 190 days. Densitometrical quantification relative to the 30-day time point and actin is given. c Top, Average 6mer seed viability of all significantly up- and downregulated miRNAs in PBMCs of 11 old versus 11 young donors⁵⁶. Bottom, Nucleotide composition at each of the six seed positions in the miRNAs analyzed above. d, Top, 6mer seed viability plots of the differential 6mer seed viability of postmortem cognitive normal (CN) and SuperAger brains. Age of the three CN participants: 100, 87, 83 - average = 90, age of the three SuperAgers: 82, 90, 85 - average = 85.7. Bottom, average 6mer seed composition of the sRNAs in the same samples. Medians (in the color of each sample/condition) and Kruskal-Wallis median test p-value are given in the box plots in a-d. For definition of box and whisker plot see legend of Fig. 1.