Fig. 6: Dependence of Aβ42-induced cell death and DNA damage on an active RISC. | Nature Communications

Fig. 6: Dependence of Aβ42-induced cell death and DNA damage on an active RISC.

From: Death Induced by Survival gene Elimination (DISE) correlates with neurotoxicity in Alzheimer’s disease and aging

Fig. 6: Dependence of Aβ42-induced cell death and DNA damage on an active RISC.The alternative text for this image may have been generated using AI.

a Viability assay of differentiated SH cells control treated for 72 hrs with either 10 μM Aβ40 or Aβ42 in the absence or presence of 10 μM ATA. Experiment was done in four technical replicates. b Western blot analysis of parental (Par) SH cells and three Ago2 k.o. clones. Experiments in a and b are representative of three independent biological repeats. c Western blot analysis of undifferentiated or 7-day differentiated SH cells and two Ago2 k.o. clones. d Viability assay of three Ago2 k.o. SH clones after exposure to 20 μM Aβ40 or Aβ42 for 72 hrs. Another k.o. clone was analyzed with similar results. This represents three independent experiments. Analysis of clones A9 and A10 is based on 5 technical replicates and of clone A11 on 8 technical replicates. P-values are given for the comparison of treated k.o. with parental cells in the same experiment. e, Top, Western blot analysis of differentiated SH cells control treated or pretreated with different concentrations of Enoxacin for 24 hrs and then treated with either 20 μM Aβ40 or Aβ42 for 24 hrs. This is representative of three independent experiments. Bottom, viability assay of the cells treated as above but for 72 hrs. This is based on 4 technical replicates. f Kinetics of H2AX phosphorylation of differentiated SH cells treated with 20 μM Aβ42. g Western blot analysis of differentiated SH cells first treated with 20 μM of Aβ40 or Aβ42 for 24 hrs and then control treated or treated with Enoxacin for 2 hours. In the sample on the right Aβ42 was washed out before an additional incubation for 2 hours. These data are representative of two independent experiments. h Western blot analysis of undifferentiated or 7-day differentiated parental SH cells or tau k.o. clone 231 K. i Viability assay of differentiated parental SH cells or two tau k.o. clones after mock treatment or treatment with 20 μM Aβ40/42. Experiment represents 4 technical replicates. This experiment was repeated with both differentiated and undifferentiated cells with similar results. j Western blot analysis of parental SH cells or different k.o. clones treated with 20 μM Aβ40/42 for 24 hrs. This is one of two independent experiments. k Western blot for γH2AX of 7 day differentiated SH cells treated with Aβ40 or Aβ42 for four hours in the absence or pretreated for 24 hrs with of 10 μM ATA. This experiment was repeated twice with different ATA concentrations. Mean with SD and two-sided Student’s t-test p-values are shown (a, d, i).

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