Fig. 4: No influence of mIgG FcG on BCR signal transduction. | Nature Communications

Fig. 4: No influence of mIgG FcG on BCR signal transduction.

From: N-linked Fc glycosylation is not required for IgG-B-cell receptor function in a GC-derived B-cell line

Fig. 4: No influence of mIgG FcG on BCR signal transduction.The alternative text for this image may have been generated using AI.

a Flow histograms showing phosphorylation of Syk (pSyk) of 2G9, 3F3, and D2 FcG+ and FcG− B cells after 5 min PBS and anti-IgG stimulation (5 μg/ml). b pSyk MFI values of 2G9, 3F3 and D2 FcG+ and FcG− B cells after 5 min stimulation. The ratio towards PBS unstimulated cells is depicted. Bar graphs show mean, standard error, and paired individual data points. n = 3 biologically independent experiments (5 µg/ml TT stim. of D2), n = 4 biologically independent experiments (5 µg/ml CCP2 stim. of 3F3 and 5 µg/ml anti-IgG stim of 3F3/D2) or n = 5 biologically independent experiments (C5 µg/ml CP2 and 5 µg/ml anti-IgG stim. of 2G9). ns (not significant) p > 0.05 (two-sided paired t tests). c pSyk MFI values of 2G9 FcG+ and FcG− B cells after 5 min stimulation with 0–25 μg/ml anti-IgG and CCP2. Representative results of two biological replicates are shown. d pSyk MFI values of 2G9 FcG+ and FcG− B cells unstimulated (us) and after 5–30 min stimulation with 5 μg/ml anti-IgG. MDL-AID KO cells are shown as control. Representative results of two biological replicates are shown. Source data are provided as a Source Data file.

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